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KMID : 0359320020420040451
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Korean Journal of Veterinary Research
2002 Volume.42 No. 4 p.451 ~ p.457
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Inadequacy of application of cytokinesis-blocked cells in fish(Rock fish, Sebastes schlegeli) and fowl(chicken) as biological dosimeter for radiation exposure
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Abstract
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The purpose of the present experiment was to investigate the micronuclei (MN) frequency in cytokinesis-blocked (CB) cells after various doses of gamma-rays in two species (fish and fowl) and so to contribute to the clarification of the question whether these species are suitable as a target organism in the test system. The frequencies of binucleated cells, and gamma-ray-induced MN in CB cells at several doses were measured in three donors of two species. No binucleated cell was noted in erythrocyte. The peaks of binucleated lymphocyte formation were found at a concentration of 2% phytohaemagglutinin (PHA) and 3 ㎍/㎖ cytochalasin B (Cyt-B) in fish at 144 hours after incubation and 2% PHA and 6 ㎍/㎖ Cyt-B in fowl at 72 hours after incubation. But the micronucleus counts failed to show any evidence of radiation damage. Measurements performed after irradiation showed a dose-related decrease in the formation of binucleated cells in each of the donors studied. Results indicated that the assays were not suitable for this due to blastization inhibition (binucleation failure) after irradiation. We conclude that the use of CB cell from fish and fowl for detecting the results of radiation exposure was highly questionable.
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